Gain Higher Resolution Across More Samples with Arima Capture-HiC+
The three-dimensional (3D) organization of chromatin can be determined through genome-wide next generation sequencing (NGS) after chromatin conformation capture (3C), a technique called Hi-C. High-resolution maps of chromatin interactions provide insight into chromatin organization and gene regulation in disease contexts. Deep sequencing and high levels of sequencing coverage are necessary to achieve the resolution necessary for chromatin studies. Achieving the level of depth and coverage required for the whole genome (whole genome sequencing) can take a lot of time, cost, and data processing power.
In these cases, Capture Hi-C can be a better alternative. Arima Capture-HiC+ combines the 3C technology of Hi-C with the targeted results of hybridization capture. This targeted next generation sequencing technique uses biotinylated probes to capture, or enrich, regions of interest, saving time and money. Targeting specific regions of the genome focuses results, lowering the barrier to generating high-resolution Hi-C data.